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目的 检测肿瘤坏死因子、白细胞介素 2、α 干扰素及合用异博定对人乳腺癌耐药细胞系的耐药性的调节作用。方法 MTT法检测药物对MCF 7/Adr细胞的抑制率变化 ,流式细胞术检测肿瘤坏死因子、白细胞介素 2、α 干扰素及合用异博定后 ,细胞内罗丹明 12 3的药物浓度变化。结果 α 干扰素可增加耐药细胞内的药物浓度 ,α 干扰素与异博定合用比单用异博定也可增加细胞内的药物浓度。各用药组均可不同程度提高阿霉素对耐药细胞的抑制率。结论 乳腺癌化疗中 ,合用α 干扰素及异博定 ,可在减少副作用的基础上逆转肿瘤的耐药性
Objective To investigate the regulatory effects of tumor necrosis factor-α, interleukin-2, interferon-α, and combination of isobrotine on drug resistance of human breast cancer cell lines. Methods The inhibitory rate of MCF 7/Adr cells was detected by MTT assay. The concentration of rhodamine 12 3 in cells was detected by flow cytometry after the detection of tumor necrosis factor, interleukin-2, interferon-α, and combination of iveteridine. . RESULTS: Alpha-interferon can increase drug concentration in drug-resistant cells. The combination of alpha-interferon and epoetin can also increase intracellular drug concentration than that of ibuprofen alone. Each drug group can improve the inhibition rate of doxorubicin to drug-resistant cells in different degrees. Conclusion In chemotherapy for breast cancer, combined use of alpha-interferon and isobromide can reverse the resistance of tumors based on the reduction of side effects.