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目的:应用微波技术处理后以高效液相色谱法测定乳癖消片中三七皂苷R1和人参皂苷Rg1的含量。方法:以水为溶剂,以微波回流的方式提取乳癖消片中三七皂苷R1和人参皂苷Rg1,制成供试品溶液,以Agilent Eclipse XDB-C18(4.6 mm×150 mm,5μm)为色谱柱,乙腈-0.05%磷酸溶液(20∶80)为流动相,流速0.72 mL.min-1,柱温20℃,检测波长203nm,参比波长360 nm,并在400～190 nm做全波长扫描。结果:在本色谱条件下,三七皂苷R1和人参皂苷Rg1可得到完全分离,三七皂苷R1的浓度在49.25～492.5μg.mL-1、人参皂苷Rg1的浓度在53.00～530.0μg.mL-1范围内与峰面积均呈良好的线性关系,相关系数均为0.9999;回收率(n=6)分别为98.19%和101.5%。结论:本方法简便、准确,重复性较好,其主要特点是快速、节能、节省溶剂、污染小,从而降低了劳动强度,改善了工作环境。
OBJECTIVE: To determine the content of notoginsenoside R1 and ginsenoside Rg1 in Ruupixiao Tablets by high performance liquid chromatography after microwave treatment. Methods: The sample of Rupiuxiao tablet was extracted with ROHS by the way of water as solvent and microwave was refluxed. The sample was prepared with Agilent Eclipse XDB-C18 (4.6 mm × 150 mm, 5 μm) as The mobile phase was acetonitrile-0.05% phosphoric acid solution (20:80), the flow rate was 0.72 mL · min-1, the column temperature was 20 ℃, the detection wavelength was 203 nm, the reference wavelength was 360 nm and the full wavelength scanning. Results: Under the chromatographic conditions, notoginsenoside R1 and ginsenoside Rg1 were completely separated, the concentration of notoginsenoside R1 was 49.25-492.5μg.mL-1, the concentration of ginsenoside Rg1 was 53.00-530.0μg.mL- 1 range and peak area showed a good linear relationship, the correlation coefficient was 0.9999; recovery (n = 6) were 98.19% and 101.5%. Conclusion: The method is simple, accurate and reproducible. The main features of the method are rapid, energy saving, solvent saving and low pollution, which reduces the labor intensity and improves the work environment.