类器官是由单个干细胞、祖细胞或不同谱系的细胞簇在三维培养条件下自发形成类似体内组织结构的细胞簇.近年来,类器官技术为干细胞和癌症领域带来了革命性的变化.在这篇综述中,我们简要地概述了前列腺类器官技术(prostate organoid technology, POT)在前列腺研究中的进展.此外,我们也总结了POT在探究前列腺干细胞和癌症生物学以及探索前列腺癌治疗策略方面的潜在应用.最后,我们就当前POT在应用过程中的技术短板进行了讨论,并为第二代POT在研究前列腺癌细胞行为和患者对药物反应中的用途提供了全新的视角.“,”Organoids are self-organized cellular clusters in three-dimensional culture,which can be derived from a single stem cell,progenitor or cell clusters of different lineages resembling in vivo tissue architecture of an organ.In the recent years,organoids technology has contributed to the revolutionary changes in stem cell and cancer fields.In this review,we have briefly overviewed the emerging landscape of prostate organoid technology (POT) in prostate research.In addition,we have also summarized the potential application of POT in the understanding of prostate stem cell and cancer biology and the discovery of novel therapeutic strategies for prostate cancer.Lastly,we have critically discussed key challenges that lie in the current state of POT and provided a future perspective on the second-generation of POT,which should better recapitulate cellular behaviors and drug responses of prostate cancer patients.