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为探讨克隆有恶性疟原虫ＭＳＰ１基因片断的重组ｐＱＥＭ１质粒在减毒鼠伤寒杆菌中的表达。使用转化和转导的方法构建减毒鼠伤寒杆菌Ｘ４０６４（ｐＲＥＰ４／ｐＱＥＭ１）；通过ＳＤＳ－ＰＡＧＥ电泳检测减毒鼠伤寒杆菌Ｘ４０６４（ｐＱＥＭ１）和减毒鼠伤寒杆菌Ｘ４０６４（ｐＲＥＰ４／ｐＱＥＭ１）的表达。实验结果成功地构建了减毒鼠伤寒杆菌Ｘ４０６４（ｐＱＥＭ１），Ｘ４０６４（ｐＲＥＰ４／ｐＱＥＭ１）的诱导型表达量高于Ｘ４０６４（ｐＱＥＭ１）的组成型表达量。结果指出不受调控的表达将降低重组ｐＱＥＭ１质粒在减毒鼠伤寒杆菌Ｘ４０６４中的表达量
To investigate the expression of recombinant pQEM1 plasmid cloned with MSP1 gene of Plasmodium falciparum in attenuated Salmonella typhimurium. Attenuated Salmonella typhimurium X4064 (pREP4 / pQEM1) was constructed using transformation and transduction methods; expression of attenuated S. typhimurium X4064 (pQEM1) and attenuated S. typhimurium X4064 (pREP4 / pQEM1) was examined by SDS-PAGE electrophoresis. Experimental Results The constitutive expression of attenuated S. typhimurium X4064 (pQEM1) and X4064 (pREP4 / pQEM1) was higher than that of X4064 (pQEM1). The results indicate that unregulated expression reduces the expression of recombinant pQEM1 plasmid in attenuated Salmonella typhimurium X4064