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本研究旨在探索旋转式细胞培养系统(RCCS)模拟微重力环境下虫草多糖促进淋巴细胞增殖和表面CD分子表达的作用。体外分离培养小鼠脾淋巴细胞,在RCCS模拟微重力环境中分别添加6.25、12.5、25和50μg/ml的虫草多糖溶液培养,在24、48和72 h检测虫草多糖对淋巴细胞增殖及表面CD分子表达的作用。结果表明,模拟微重力环境抑制了淋巴细胞的增殖能力,25和50μg/ml虫草多糖对淋巴细胞的增殖和CD4、CD8的表达均具有促进作用,但50μg/ml虫草多糖促进淋巴细胞增殖的作用随时间延长变为抑制作用。结论:适宜浓度范围内的虫草多糖具有对抗模拟微重力环境下淋巴细胞增殖能力下降和表面CD分子表达减少的作用,为筛选对抗失重效应、促进免疫力的药物提供理论依据和实验基础。
This study aimed to explore the role of Cordyceps polysaccharide in promoting lymphocyte proliferation and surface CD expression in a rotating cell culture system (RCCS) simulating microgravity. Splenic lymphocytes were isolated and cultured in vitro. Cordyceps polysaccharides solution of 6.25, 12.5, 25 and 50μg / ml were respectively cultured in simulated microgravity environment of RCCS. Cordyceps polysaccharides on lymphocyte proliferation and surface CD were detected at 24, 48 and 72 h The role of molecular expression. The results showed that the simulated microgravity environment inhibited the proliferation of lymphocytes, and the effects of 25 and 50μg / ml Cordyceps polysaccharides on the proliferation of lymphocytes and the expression of CD4 and CD8 were promoted. However, the effect of 50μg / ml Cordyceps polysaccharide on lymphocyte proliferation As time goes on into inhibition. CONCLUSION: Cordyceps polysaccharide has the potential to resist lymphocyte proliferation and decrease of CD expression in simulated microgravity environment, and provide a theoretical basis and experimental basis for the screening of drugs against weightlessness and immune enhancement.