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以胆木(Nauclea officinalis)无菌实生苗的茎段、叶片、叶柄作为外植体,对其进行组织培养和快速繁殖研究。结果表明:种子消毒采用70%的酒精浸泡10 s,再用2%的次氯酸钙消毒8～10 min,效果较理想;以带芽茎段为外植体可实现丛生芽增殖;最佳丛生芽苗的诱导和增殖培养基为1/2 WPM附加0.5 mg/L 2,4-D,其次为1/2 WPM附加0.5 mg/L IBA和1/2 WPM附加1.0 mg/L IBA+0.5 mg/L 6-BA;芽苗增值系数达4.8,芽苗诱导率达94%;芽苗生根壮苗培养中效果较好的培养基为(1/2～1/4)WPM+0.5 mg/L 2,4-D和(1/2～1/4)WPM+0.5 mg/LIBA。胆木试管苗移栽成活率达到80%。
The stems, leaves and petioles of sterile seedlings of Nauclea officinalis were used as explants for their tissue culture and rapid propagation. The results showed that: seed disinfection with 70% alcohol soaked for 10 s, and then disinfected with 2% calcium hypochlorite 8 ~ 10 min, the effect is better; with stem buds as explants can achieve proliferation of tufted buds; the best The inducement and proliferation medium for shoot budding was 1/2 WPM supplemented with 0.5 mg / L 2,4-D, followed by 1/2 WPM with additional 0.5 mg / L IBA and 1/2 WPM plus 1.0 mg / L IBA + 0.5 mg / L 6-BA. The value-added coefficient of buds reached 4.8, and the induction rate of buds was 94%. The medium with better effect of rooting and sprouting in shoots was (1/2 to 1/4) WPM + 0.5 mg / L 2,4-D and (½ to ¼) WPM + 0.5 mg / L IBA. Seedling transplanting survival rate of 80%.