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以乙型肝炎病毒 (HBV)前C/C基因异质性来探讨在慢性感染者体内是否存在HBV准种。以中国株HBV基因序列为依据 ,设计特异性多聚酶链反应 (PCR)引物 ,自 4例慢性HBV感染患者血清中扩增HBV前C/C基因序列 ,克隆入pGEMTeasy载体 ,每例患者挑选 5个克隆测序以比较病毒的变异程度。测序结果发现同一患者前C/C基因碱基序列之间的同源性大于 98% ,但存有G83→A替换、C抗原内部缺失、移框突变等多种变异类型。结果提示 ,HBV长期携带者体内有HBV准种共存 ,推测前C/C区的多种变异可能与感染慢性化有关。
Hepatitis B virus (HBV) pre-C / C gene heterogeneity to explore the presence of HBV quasispecies in chronically infected individuals. Based on the Chinese strain HBV gene sequence, a specific polymerase chain reaction (PCR) primer was designed and the pre-HBV C / C gene sequence was amplified from the serum of 4 patients with chronic HBV infection and cloned into pGEMTeasy vector. Each patient was screened for 5 Clone sequencing to compare the degree of variation of the virus. Sequencing results showed that there was more than 98% homology between the base sequences of pre-C / C genes in the same patient, but there were many types of mutation, such as G83 → A substitution, C antigen internal deletion and frame-shifting mutation. The results suggest that HBV long-term carriers of HBV quasispecies coexist, suggesting that a variety of pre-C / C mutations may be related to chronic infection.