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5-羟色胺(5-hydroxytryptamine,5-HT)存在于各种动物的消化道中,并影响甲壳动物的生长和繁殖。色氨酸羟化酶是5-HT生物合成中的限速酶,可作为5-HT免疫活性细胞的特异标志。运用组织学和免疫组织化学技术对中华绒鳌蟹中肠、肠球和后肠的形态学及色氨酸羟化酶在其中的分布进行研究,结果显示:中华绒螯蟹肠道中肠和后肠组织结构组成基本相似,均由黏膜层、黏膜下层、肌层和外膜四部分组成。然而,肠球外膜非常发达,由结缔组织组成。其余组织形态一部分与中肠相似(近头端),另一部分与后肠相似(近尾端)。色氨酸羟化酶在中华绒螯蟹中肠、肠球和后肠均有分布,阳性物质呈棕褐色。在中肠,主要分布在黏膜上皮细胞的胞核中;在肠球,主要分布在近中肠端黏膜上皮细胞的胞核中、近后肠端的黏膜上皮细胞的胞浆中、以及外周结缔组织中;在后肠,主要分布在黏膜上皮细胞的胞浆中。为进一步了解5-HT在甲壳动物消化道中的生理调节机制奠定了基础。
5-hydroxytryptamine (5-HT) is present in the digestive tract of various animals and affects the growth and reproduction of crustacean. Tryptophan hydroxylase is the rate-limiting enzyme in 5-HT biosynthesis and serves as a specific marker for 5-HT immunocompetent cells. Histology and immunohistochemical techniques were used to study the morphology and the distribution of tryptophan hydroxylase in the midgut, intestine and hindgut of the Chinese crab Eriocheir sinensis. The results showed that: Intestinal tissue structure is basically similar, by the mucosa, submucosa, myometrium and outer membrane composed of four parts. However, the enteroepithelial membrane is well developed and consists of connective tissue. The rest of the morphology of the part of the midgut similar (near head), the other part of the hindgut similar (near the tail). Tryptophan hydroxylase in the mitten crab midgut, enterococci and hindgut distribution, the positive substance was brown. In the midgut, the main distribution in the mucosal epithelial cells in the nucleus; in the intestine, mainly in the proximal midgut mucosal epithelial cells in the nucleus, mucosal epithelial cells near the end of the intestinal cytoplasm, and peripheral connective tissue In the hindgut, mainly in the cytoplasm of mucosal epithelial cells. Which laid the foundation for understanding the physiological regulation mechanism of 5-HT in the digestive tract of crustacean.