论文部分内容阅读
目的 研究弓形虫感染对雄性小鼠睾丸组织细胞周期的影响。方法 应用流式细胞术检测弓形虫感染10 3/ ml、10 4 / ml、10 6 / ml3个剂量组小鼠睾丸组织细胞周期,并设立生理盐水和环磷酰胺对照组。结果 不同剂量的弓形虫感染均可使小鼠睾丸组织细胞各时相的细胞百分数发生变化,并随着剂量的增加,G0 / G1 、S时相的细胞百分数明显减少,与阴性对照组比较差异有显著性(P<0 .0 5 )。G2 / M时相的细胞百分数逐渐增多,各剂量组与阴性对照组比较差异有显著性(P<0 .0 5 )。结论 弓形虫感染可能抑制小鼠睾丸细胞的DNA合成,可引起G2 期细胞阻滞,从而使细胞的有丝分裂延伸。
Objective To study the effect of Toxoplasma gondii infection on testicular cell cycle in male mice. Methods Flow cytometry was used to detect the cell cycle of testis in mice of 10 3 / ml, 10 4 / ml and 10 6 / ml Toxoplasma gondii infection, and normal saline and cyclophosphamide control group were established. Results Toxoplasma gondii infection at different dosages could change the percentage of cells in each phase of testicular cells in mice. With the increase of dosage, the percentage of cells in G0 / G1 and S phase decreased obviously, which was significantly lower than that in the negative control group There was significant (P <0.05). G2 / M phase cells gradually increased the percentage of each dose group and negative control group, the difference was significant (P <0. 05). Conclusion Toxoplasma gondii infection may inhibit the DNA synthesis in mouse testis cells, which may lead to cell arrest in G2 phase, which leads to mitotic extension of cells.