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通过旱育秧的方式进行水稻育秧,测定秧苗根系和叶片电导率、叶绿素、丙二醛(MDA)及抗氧化酶的变化,研究稻田土(S)、自然堆沤稻草+稻田土(SS)、加复合腐解菌剂堆沤稻草+稻田土(SMS)3种育秧基质对水稻生长和抗性的影响.结果发现:在20 d时,SMS处理组电导率最大,为41.2(±0.3)μS/cm,叶片MDA含量是S处理组的1.93倍,叶片多酚氧化酶(PPO)酶活比S、SS处理组分别提高了56.15%、8.96%,根系过氧化物酶(POD)酶活比S、SS处理分别提高了226.04%、63.16%,根系苯丙氨酸解氨酶(PAL)酶活为178.79(±4.02)U g~(-1) min~(-1);40 d时,SMS处理组的根系和叶片PAL酶活比S处理组分别提高了135.28%和148.32%.因此,加复合腐解菌剂堆沤稻草作为育秧基质促进了秧苗生长,显著提高了秧苗的抗性.
The rice seedlings were planted by drought-nursery seedlings, and the changes of conductivity, chlorophyll, malondialdehyde (MDA) and antioxidant enzyme activities in root and leaf of the seedlings were measured. The effects of paddy soil (S) The results showed that the conductivity of SMS treated group was the highest at 41 d (± 0.3) μS / cm, the content of MDA in leaves was 1.93 times of that of S, and the activity of PPO increased 56.15% and 8.96% respectively compared with those of S and SS. The activity of peroxidase (POD) The activities of PAL and PAL in roots increased by 226.04%, 63.16%, 178.79 ± 4.02 U g -1 min -1, respectively. At 40 days, The PAL activity of root and leaf of SMS treatment group increased by 135.28% and 148.32%, respectively, compared with S treatment group. Therefore, using composting composting agent as straw seedling substrate promoted the growth of seedling and significantly increased the seedling resistance.