介绍了生物杀虫剂阿维菌素的液相色谱分析方法。以甲醇 +乙腈 +水为流动相 ,使用以WatersNova-PakC18为填料的不锈钢柱和紫外检测器 (2 4 5nm)。对阿维菌素原药、乳油试样中的阿维菌素进行反相高效液相色谱分离 ,外标法定量。其线性相关系数为 0 .9999,测定原药、乳油回收率分别在 99.2 %～ 10 0 .9%和98.7%～ 10 1.4 %之间 ,标准偏差分别为 0 .32、0 .0 0 89,变异系数分别小于 0 .34%、1.1% The method of liquid chromatographic analysis of avermectins for biological pesticides was introduced. Using methanol + acetonitrile + water as the mobile phase, a stainless steel column packed with Waters Nova-Pak C18 and a UV detector (245 nm) were used. Avermectin original drug, EC sample of avermectin RP-HPLC, external standard. The linear correlation coefficient was 0.9999. The recoveries of the original drugs and the emulsifiable concentrates were between 99.2% -109.0% and 98.7% -10.4%, respectively. The standard deviations were 0 .32,0. 0 0 89, The coefficient of variation was less than 0.34%, 1.1%