目的:以常规体检者和明确诊断的大肠癌患者为研究对象,对其血清进行多肽谱分析,统计分析获得大肠癌特异血清多肽峰,为大肠癌的分子诊断提供理论依据,提高大肠癌的早期诊断水平。方法:1)收集研究对象外周非抗凝血并记录其人口学特征,将非抗凝血进行离心分离血清并保存;2)用Dynabeads RPC18磁珠分离提取血清蛋白质,Bruker UltraFlex TOF/TOF采集信号并用分析软件Clinprot tools 2.2(Bruker)分析筛选出大肠癌血清显著差异峰;3)用SPSS13.0分析大肠癌患者和健康人多肽峰的差异,进行Logistic回归分析差异多肽对形成大肠癌的影响。结果:本次研究共获得111名健康人和94名大肠癌患者的血清多肽峰信息,其中109名健康人和91名大肠癌患者同时具有性别、年龄等人口学信息。筛选出差异多肽峰105个,其中76个多肽在大肠癌患者和健康人间的分布差异有统计学意义(P<0.05)。运用Logistic回归分析,进入回归方程(P<0.05)的有:年龄,质荷比(m/z)分别为1061.10、1213.09、1607.32、1867.02、1897.95、2011.67和5078.81的七种多肽。结论:液体蛋白芯片飞行时间质谱系统可高效、精确地筛查血清多肽。大肠癌患者与健康人的血清多肽存在差异,筛选得到的质荷比(m/z)分别为1061.10、1213.09、1607.32、1867.02、1897.95、2011.67和5078.81的七种多肽可能作为早期诊断大肠癌的潜在肿瘤标志物。 OBJECTIVE: To study the polypeptide spectrum of serum in patients with routine physical examination and definite diagnosis of colorectal cancer, and to obtain the serum-specific polypeptide peak by statistical analysis to provide a theoretical basis for the molecular diagnosis of colorectal cancer and to improve the early stage of colorectal cancer Diagnostic level. Methods: 1) Non-anticoagulated peripheral blood was collected and its demographic characteristics were recorded. Serum proteins were separated by centrifugation and stored in non-anticoagulated blood. 2) Serum proteins were separated and extracted with Dynabeads RPC18 magnetic beads. Bruker UltraFlex TOF / TOF signals And analyzed by Clinprot tools 2.2 (Bruker). The results showed that there was significant difference in the serum of colorectal cancer by using the analysis software Clinprot tools 2.2 (Bruker). 3) Using SPSS 13.0 to analyze the differences of peptide peaks between patients with colorectal cancer and healthy people, Logistic regression analysis was used to analyze the effect of different peptides on the formation of colorectal cancer. Results: In this study, a total of 111 healthy people and 94 patients with colorectal cancer serum peptide peak information, of which 109 healthy people and 91 patients with colorectal cancer both sex, age and other demographic information. The results showed that there were 105 polypeptide peaks among which 76 peptides were statistically different between colorectal cancer patients and healthy individuals (P <0.05). Logistic regression analysis showed that there were seven polypeptides with the age and mass-to-charge ratio (m / z) of 1061.10, 1213.09, 1607.32, 1867.02, 1897.95, 2011.67 and 5078.81, respectively. Conclusion: The liquid protein chip time of flight mass spectrometry system can screen serum peptides efficiently and accurately. Serum peptides of patients with colorectal cancer were different from those of healthy individuals. The m / z values ​​of the peptides were 1061.10, 1213.09, 1607.32, 1867.02, 1897.95, 2011.67 and 5078.81, respectively, which might be potential candidates for early diagnosis of colorectal cancer Tumor markers.