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目的:采用高效液相色谱法建立六味地黄浓缩丸的特征图谱,并同时测定4种主要成分的含量。方法:以没食子酸、马钱苷、芍药苷、丹皮酚为对照,采用Kromasil C18柱(250 mm×4.6 mm,5μm),乙腈-0.015%磷酸溶液梯度洗脱,流速1.0mL·min-1,检测波长238 nm,柱温35℃进行试验,用国家药典委员会“中药色谱指纹图谱相似度评价系统2004A”处理分析。结果:不同厂家的六味地黄浓缩丸特征图谱相似度较高,但没食子酸、马钱苷、芍药苷、丹皮酚含量差异较大。结论:该方法快速简便、准确可靠、重复性好,六味地黄浓缩丸特征图谱专属性强,可结合4种主要成分的含量测定全面控制六味地黄浓缩丸的质量,为六味地黄浓缩丸的质量评价提供科学依据。
OBJECTIVE: To establish the characteristic map of Liuwei Rehmannia glutinosa pellets by high performance liquid chromatography (HPLC) and determine the content of four main components simultaneously. Methods: Gallic acid, loganin, paeoniflorin and paeonol were used as control. The elution was performed on a Kromasil C18 column (250 mm × 4.6 mm, 5 μm) with a gradient of acetonitrile-0.015% phosphoric acid. The flow rate was 1.0 mL · min -1 , The detection wavelength of 238 nm, the column temperature of 35 ℃ for testing, with the State Pharmacopoeia Commission “Chinese medicine chromatographic fingerprint similarity evaluation system 2004A ” analysis. Results: The similarity of the features of the six-flavor rehmannia pills from different manufacturers was high, but there were significant differences in gallic acid, loganin, paeoniflorin and paeonol content. Conclusion: The method is rapid, simple, accurate, reliable and reproducible. The characteristic map of Liuwei Dihuang Pills is special and can be used to control the quality of Liuweidihuang Pills comprehensively. It can be used to evaluate the quality of Liuwei Dihuang Pills Provide a scientific basis.