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本文报道了预免疫b型流感杆菌(PRP)和白喉类毒素(DT)偶联菌苗对人B淋巴细胞应答的影响,用25μg PRP和18μg DT共价结合的偶联菌苗(PRP-D)对32名成人志愿者接种1或2次。在免疫前(0天)和免疫后4周,收集血样测定抗体浓度,在免疫后的第6、7、8、9天采血测定分泌抗体细胞(AbSC)数,用溶血斑形成法和ELISA法分别测定B细胞的分泌能力和抗体水平,结果如下。健康成人首次接种PRP-D偶联菌苗后4周,血浆中PRP的IgG、IgM和IgA的抗体总量的增长为32.0μg/ml,DT的抗体总量的增长为1.14IU/ml,但其中IgM抗体未增长,同免疫前基本一致。免疫后除1人外在6～9天时PRP和DF的血循AbSC的数目均增加。其中IgA的AbSC的数目占IgG和IgM的一半以上,DT的AbSC主要为IgG。
This paper reports the effect of PRB and DT conjugate vaccines on human B lymphocyte responses using a conjugate vaccine (PRP-D) covalently conjugated with 25 μg PRP and 18 μg DT ) Thirty-two adult volunteers were vaccinated 1 or 2 times. Blood samples were collected before antibody immunization (Day 0) and 4 weeks after immunization. Antibody concentrations were measured. Blood samples were collected at 6, 7, 8 and 9 days after immunization. The number of secreted antibody (AbSC) cells was determined by hemolytic plaque assay and ELISA Respectively determine the secretion of B cells and antibody levels, the results are as follows. At 4 weeks after the first immunization of PRP-D conjugate vaccine in healthy adults, the total amount of IgG, IgM and IgA antibodies in plasma increased by 32.0 μg / ml and the total antibody titer in DT increased by 1.14 IU / ml, Which IgM antibody did not grow, with the pre-immune basically the same. The numbers of AbSCs in the bloodstream of PRP and DF increased after 6 to 9 days except one after immunization. Among them, the number of AbSC of IgA accounted for more than half of IgG and IgM, and the AbSC of DT was mainly IgG.