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目的:观察益气扶正方对Lewis肺癌小鼠肿瘤生长的影响,并通过拆方和不同时间点预防性给药研究其抑瘤作用。方法:选用5周龄纯系C57BL/6雄性小鼠150只,将Lewis肺癌细胞悬液接种于小鼠右侧腋下建立皮下肺癌模型,接种后小鼠随机分组,治疗组给予相应中药汤剂灌胃,对照组给予同体积生理盐水,测量肿瘤体积大小并统计。结果:益气扶正方能抑制Lewis肺癌皮下移植瘤小鼠的肿瘤生长(P<0.05),拆方后的加减四君子汤和黄芪、党参、白术、茯苓、陈皮均未观察到抑瘤作用。益气扶正方接种前2周、接种前1周、接种后给药均能发挥抑瘤作用(P<0.05)。结论:益气扶正方能明显抑制Lewis肺癌皮下移植瘤小鼠的肿瘤生长,预防性给药则起效更早,抗癌作用更显著。
OBJECTIVE: To observe the effect of Yiqi Fuzheng Decoction on tumor growth in mice with Lewis lung cancer and to study its antitumor effect by demolition prescription and prophylactic administration at different time points. Methods: Fifty-five-week-old C57BL / 6 male mice were selected. Lewis lung cancer cell suspension was inoculated into the right armpit of mice to establish a subcutaneous lung cancer model. After inoculation, the mice were randomly divided into groups. The treatment group was given the corresponding Chinese medicine decoction Gavage, the control group were given the same volume of saline, the size of the tumor measurements and statistics. Results: Yiqi Fuzheng Decoction can inhibit tumor growth in Lewis lung cancer subcutaneously transplanted mice (P <0.05). No anti-tumor effect was observed after addition and subtraction of Sijunzi Decoction and Astragalus mongholicus, Codonopsis pilosula, Atractylodes macrocephala, Poria cocos and Tangerine peel. Yiqi Fuzheng 2 weeks before vaccination, 1 week before inoculation, after inoculation can exert antitumor effect (P <0.05). Conclusion: Yiqi Fuzheng Decoction can significantly inhibit tumor growth in Lewis lung cancer subcutaneously transplanted mice, while prophylactic administration is more effective and anti-cancer effect is more significant.