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目的研究异丙酚对大鼠脑缺血再灌注损伤的保护作用及其机制。方法SD大鼠90 只,体重290-310 g,随机分成3组,异丙酚组(P组,n=42);生理盐水组(NS组,n=42);假手术组(S 组,n=6)。NS、P组采用线栓法(尼龙线栓插入颈外动脉)制备局灶性脑缺血再灌注损伤模型,P组在缺血前10min腹腔注射异丙酚10mg/100 g,NS组给予等量生理盐水;S组只作切口,不作颈总动脉插线。P和NS组分别在再灌注即刻、2 h、5 h、11 h、23 h、71 h、1周,处死6只大鼠,断头取脑,S组于再灌注11 h断头取脑,制作脑片,计算脑梗塞体积比;测定缺血半影区GLUT1 mRNA及蛋白表达水平。结果与S组相比,P、NS组再灌注各时点脑梗塞体积比增大;P组再灌注各时点梗塞体积小于NS组(P<0.05或0.01)。P、NS组缺血半影区GLUT1 mRNA及蛋白表达从再灌注即刻开始升高,23 h达高峰;P组再灌注各时点缺血半影区均高于NS组,P、NS组GLUT1 mRNA及蛋白表达再灌注各时点均高于S组(P<0.05或0.01)。结论异丙酚对大鼠缺血再灌注损伤有一定保护作用,上调缺血半影区GLUT1的表达是其机制之一。
Objective To investigate the protective effect of propofol on cerebral ischemia-reperfusion injury in rats and its mechanism. Methods Ninety SD rats weighing 290-310 g were randomly divided into 3 groups: propofol group (n = 42), NS group (n = 42), sham operation group (group S, n = 6). The model of focal cerebral ischemia / reperfusion injury was established in rats in NS and P groups by thread suture (nylon thread inserted into external carotid artery). In group P, propofol 10 mg / 100 g The amount of saline; S group only for incision, not for common carotid artery. Six rats were sacrificed immediately after reperfusion, 2 h, 5 h, 11 h, 23 h, 71 h and 1 wk respectively in the P and NS groups. The rats in the S group were decapitated and the brain was decapitated at 11 h after reperfusion , Making brain slices, calculating the volume ratio of cerebral infarction; measuring the expression of GLUT1 mRNA and protein in ischemic penumbra. Results Compared with group S, the volume of cerebral infarction increased at each time point in reperfusion group in P and NS groups. The volume of infarction in group P was lower than that in NS group at each time points (P <0.05 or 0.01). The expression of GLUT1 mRNA and protein in ischemic penumbra of P and NS groups increased immediately after reperfusion and peaked at 23 hours. The ischemic penumbra in group P at each time point was higher than that in NS group. The GLUT1 The expression of mRNA and protein at each time point was higher than that in S group (P <0.05 or 0.01). Conclusion Propofol has a protective effect on ischemia-reperfusion injury in rats. Up-regulation of GLUT1 expression in ischemic penumbra is one of the mechanisms.