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目的 获得具有生物学活性的重组蛋白，用于制备抗－ＨＥＶ酶标试剂盒。方法 将插入的含ＨＥＶ 部分开放读码框２（ＯＲＦ２）的重组菌扩大培养，诱导表达，纯化，鉴定，组装酶标试剂盒。结果 纯化的重组蛋白经 ＳＤＳ－ＰＡＧＥ鉴定，在相对分子质量５６０００处有一明显的蛋白带，经ＣＳ－９３０薄层扫描纯度达９５％以上，经免疫印迹 分析，具有良好的免疫学特异性。此ＨＥＶ试剂盒，检测阳性病人血清样品阳性率为９４．７４％，检献血员血清样品阳 性率为５．４３％。结论 该纯化的ＨＥＶ部分ＯＲＦ２重组蛋白组装的试剂盒特异性及敏感性均较好。
Objective To obtain a biologically active recombinant protein for the preparation of anti-HEV enzyme labeling kit. Methods The inserted recombinants containing open reading frame 2 (ORF2) of HEV were amplified and induced to express, purify, identify and assemble ELISA kit. Results The purified recombinant protein was identified by SDS-PAGE and had a clear protein band at a molecular weight of 56000. The purity of the purified recombinant protein was over 95% by scanning electron microscopy with a CS-930 thin-layer chromatography. The purified recombinant protein showed good immunological specificity. The positive rate of serum samples of this HEV kit, positive patients was 94.74%, positive rate of serum samples of blood donors was 5.43%. Conclusion The specificity and sensitivity of the purified HEV ORF2 recombinant protein assembly kit are good.