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目的观察完全弗氏佐剂(CFA)外周刺激后,大鼠中脑导水管周围灰质(PAG)中胶质纤维酸性蛋白(GFAP)、小胶质细胞标记物(Iba)以及细胞因子IL-β、TNF-α的表达变化。方法结合行为学检测,反转录-聚合酶链式反应(RT-PCR)、Western blotting以及免疫组织化学的方法,观察了大鼠后爪注射CFA后疼痛感觉的变化,并检测了PAG中上述标记物和细胞因子在基因水平和蛋白水平的表达变化。结果注射CFA后,注射侧后爪出现热痛过敏和机械性触诱发痛;大鼠的热痛过敏14d基本恢复正常,但机械性触诱发痛持续到21d时仍未恢复正常;星形胶质细胞标记物GFAP在急性期和慢性期有显著增加;小胶质细胞的标记物CD14、细胞因子(IL-β、TNF-α)在急性期、亚急性期和慢性期均有增加。结论小胶质细胞的激活可能与炎性疼痛的起始相关,而星形胶质细胞可能与疼痛的维持相关,细胞因子表达的增加可能对痛觉过敏的发生起重要作用。
Objective To observe the changes of glial fibrillary acidic protein (GFAP), microglial marker (Iba), and cytokines IL-β in periaqueductal gray (PAG) after complete Freund’s adjuvant (CFA) , TNF-α expression changes. Methods The changes of pain sensation after the injection of CFA into rat hindpaw were observed by behavioral testing, reverse transcription - polymerase chain reaction (RT - PCR), Western blotting and immunohistochemistry. Changes in the expression of markers and cytokines at the gene and protein levels. Results After injection of CFA, thermal hyperalgesia and mechanical tactile allodynia occurred on the hindpaw of the injected side. The heat hyperalgesia in rats returned to normal on the 14th day, but the mechanical tingling pain did not return to normal after 21 days. The astrogliosis The cell marker GFAP increased significantly in both acute and chronic phase. The microglial markers CD14, cytokines (IL-β, TNF-α) increased in acute phase, subacute phase and chronic phase. Conclusion The activation of microglia may be related to the initiation of inflammatory pain. Astrocytes may be related to the maintenance of pain. Increased cytokine expression may play an important role in the development of hyperalgesia.