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本文介绍了ＤＤＲＴ－ＰＣＲ成熟相关基因分离与克隆方法的基本原理、步骤和优点；分析了该方法在非跃变型果实成熟的分子生物学机理研究中的应用。
This paper introduces the basic principles, steps and advantages of DDRT-PCR related gene isolation and cloning method. The application of this method in the molecular mechanism of non-clutter fruit ripening is analyzed.