采用SephadexG 75和HiPrep 1 6 / 6 0DEAE离子交换等方法从赤子爱胜蚓 (Eiseniafoetida)中提取到一组活性蛋白质成分 ,双向电泳分析证明它们为一组pI在 3 .0～ 4 .0之间的酸性蛋白质 ;利用体外K5 6 2、HeLa、SY5Y等肿瘤细胞抑制实验和纤维蛋白平板实验 ,跟踪测定活性 ,证明乙醇沉淀组分D2 (8)是既具肿瘤抑制 ,又具有激酶活性的蛋白质成分。同时应用非变性电泳对乙醇沉淀组分进行分离 ,并利用电洗脱、凝胶原位酶解和ESI MS等蛋白质组学方法 ,鉴定了其中 6种蛋白质的分子量、氨基酸组成、N末端序列和肽质量指纹图信息 ,其中条带 9与D2 (8)组分为同一种蛋白质 ;研究证明蚯蚓中含有既具抗肿瘤活性又具有激酶活性的蛋白质成分。采用的方法可适用于活性蛋白质成分的整体分离与鉴定 A group of active protein components was extracted from Eisenia foetida using SephadexG 75 and HiPrep 16/60 DEAE ion exchange methods. Two-dimensional electrophoresis analysis demonstrated that they were a group of pI between 3.0 and 4.0. The acid protein was detected by using K562, HeLa, SY5Y and other tumor cell inhibition experiments and fibrin plate experiments to determine the activity of the ethanol fraction D2 (8) as a protein component with both tumor suppression and kinase activity. . Simultaneously, non-denaturing electrophoresis was used to separate ethanol-precipitated fractions, and the molecular weight, amino acid composition, and N-terminal sequence of the six proteins were identified using proteomics methods such as electroelution, in-situ gel digestion and ESI MS. Peptide mass fingerprinting information, in which band 9 and D2 (8) components are the same protein; studies have shown that wolfberry contains protein components that have both anti-tumor activity and kinase activity. The method used can be applied to the overall isolation and identification of active protein components