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建立了快速分离和检测引起仔猪腹泻的肠毒性大肠杆菌K88、K99和 987P细菌细胞的毛细管区带电泳方法 ,并进行了腹泻仔猪粪便中肠毒性大肠杆菌的应用检测分析。结果表明 ,在电泳缓冲液为 0 0 5mol/LNa2 CO3 NaHCO3(pH 9 9)、分离电压为 1 4 1kV、检测波长为 2 1 0nm的电泳条件下 ,E coliK88、K99和 987P的细胞分别具有单一、稳定的特征谱峰 ,其保留时间的相对标准偏差RSD≤ 0 9% ;在确定的实验条件下 ,实现了腹泻仔猪粪便中肠毒性大肠杆菌的快速检测分析 ,发现将出生 5d～ 6d的腹泻仔猪粪便引入培养基中增殖后主要检出K88,将出生 30d～ 35d的腹泻仔猪粪便引入培养基中增殖后主要检出K99,将出生 60d左右的腹泻仔猪粪便引入培养基中增殖后未发现肠毒性大肠杆菌。
A rapid capillary zone electrophoresis method was established for the rapid separation and detection of enterotoxigenic Escherichia coli K88, K99 and 987P bacterial cells in piglets causing diarrhea. The detection and analysis of enterotoxigenic Escherichia coli in diarrhea piglets was also carried out. The results showed that the cells of E coli K88, K99 and 987P cells were single, respectively, under the conditions of electrophoresis buffer of 0 0 5mol / L Na 2 CO 3 NaHCO 3 (pH 9 9), separation voltage of 114 kV and detection wavelength of 210 nm. , Stable characteristic peak, the relative standard deviation of retention time was RSD≤0 9%. Under certain experimental conditions, rapid detection and analysis of enterotoxigenic Escherichia coli in diarrhea piglets feces was achieved. It was found that diarrhea of 5d ~ 6d The piglets excreted into the culture medium were mainly detected in the proliferation of K88, piglets from the diarrhea piglets born 30d ~ 35d were mainly introduced into the culture medium, and K99 was mainly detected in the culture medium. Toxic E. coli.