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以甜樱桃‘雷尼’为试材,利用桃基因组设计引物克隆获得两个ORF分别为519和516 bp的TFL1基因全长序列,分别编码172和171个氨基酸,均具有保守的PEBP结构域和9个底物结合位点,符合TFL1家族基因的典型特征,分别命名为Pa TFL1a和Pa TFL1b。对‘雷尼’×2121杂交组合3年生和4年生实生后代调查发现其始花节位为28.72±1.34,童程为(63.33±3.19)cm;实时荧光定量PCR分析表明其新梢顶端分生组织中,童区内Pa TFL1a和Pa TFL1b表达量远高于成年区,且成年区内的表达量随着节位增高而增加;对1~4年生实生后代不同枝龄的顶端分生组织中Pa TFL1a和Pa TFL1b表达量检测发现随树龄和枝龄的上升其表达量下调明显。因此,Pa TFL1a和Pa TFL1b基因与甜樱桃童期向成年阶段转变相关。在第30节位新梢成熟叶片中Pa TFL1a表达量不随树龄的改变而变化,而Pa TFL1b的表达与顶端分生组织中规律一致,可以作为甜樱桃‘雷尼’阶段转变过程的检测标记。
Two full-length sequences of TFL1 gene with 519 and 516 bp ORFs were cloned using peach genomic primers and encoded 172 and 171 amino acids, respectively. All of them had conserved PEBP domains and Nine substrate binding sites, in line with the typical characteristics of the TFL1 family of genes, were named Pa TFL1a and Pa TFL1b. The offspring of 3-year-old and 4-year-old offspring of ’Raney’ × 2121 hybrids were found to have a flower node of 28.72 ± 1.34 and a child path of (63.33 ± 3.19) cm. Real-time PCR analysis showed that the shoot apical meristem In the tissues, the expression levels of Pa TFL1a and Pa TFL1b in the children’s area were much higher than those in the adult area, and the expression level in the adult area increased with the increase of the node size. In the apical meristems with different ages of 1 to 4 years Pa TFL1a and Pa TFL1b expression levels detected with the rise of age and age of its expression was significantly down-regulated. Therefore, the Pa TFL1a and Pa TFL1b genes are associated with the transition from sweet cherry to adulthood. The expression level of Pa TFL1a did not change with age in the mature shoots at the 30th shoot stage, while the expression of Pa TFL1b was consistent with that in the apical meristem, which could be used as the detection marker for the ’Raney’ stage of sweet cherry.