论文部分内容阅读
本研究对72份制干辣椒种质资源采用SRAP和SCoT分子标记技术进行遗传多样性研究。以期从分子水平上揭示新疆制干辣椒种质资源间的亲缘关系,为其种质搜集、利用和遗传改良提供一定的理论基础。结果表明,在10对SRAP引物组合共检测出78个位点,其中多态性位点数69个,多态性比例为88.64%,平均每条引物扩增位点为7.8个,PIC为0.819;20对SCoT引物扩增出203个位点,其中多态性位点数174个,多态性比例为85.71%,平均每条引物扩增位点为10.15个,PIC为0.861。UPGMA聚类结果显示,在相似性系数为0.74时,72份材料可被分为2个类群;阈值为0.79时类群2又可分为5组,表明制干辣椒种质间具有相对丰富的遗传多样性。分析结果将有助于更好地保护和利用制干辣椒种质资源,研究结果为进一步进行制干辣椒的种质研究及利用提供了帮助。
In this study, genetic diversity of 72 dried pepper germplasm resources was studied using SRAP and SCoT molecular markers. In order to reveal the genetic relationship between dried apricot germplasm resources in Xinjiang at the molecular level and provide some theoretical basis for its germplasm collection, utilization and genetic improvement. The results showed that in 10 pairs of SRAP primer combinations, 78 loci were detected, of which 69 were polymorphic, with a polymorphism ratio of 88.64%. The average number of primers per primer was 7.8 and PIC was 0.819. 20 pairs of SCoT primers amplified 203 loci, of which 174 polymorphic loci, the proportion of polymorphisms was 85.71%, the average of each primer amplification site was 10.15, PIC 0.861. UPGMA clustering results showed that at the similarity coefficient of 0.74, 72 materials could be divided into two groups. When the threshold value was 0.79, the group 2 could be divided into five groups, which indicated that there were relatively rich inheritance among the cultivars Diversity. The results of the analysis will help to better protect and utilize the germplasm resources of dried chilli. The research results will be helpful for the further research and utilization of the dried chilli germplasm.