论文部分内容阅读
从3个湿地松苗圃土壤中分离出了66株松苗猝倒病的致病真菌,63个菌株为镰孢属真菌,2个为链格孢菌,1个为丝核菌属真菌。对66个真菌菌株进行松苗猝倒病的致病性测定表明:12个真菌菌株为无致病性或弱致病性真菌,17个镰孢属菌株为强致病性真菌,其余37个为中等程度致病性真菌。从湿地松幼苗根系分离到了细菌菌株320株,平皿拮抗测定表明其中的10个菌株对松苗猝倒病病原真菌有强拮抗作用,对松苗猝倒病有较大的生防潜力。16SrDNA序列分析表明这10个生防菌株的分类地位分别是:7株为洋葱伯克霍尔德菌,2株为枯草芽孢杆菌,1株为待定种。
Sixty-six plant disease-causing fungi were isolated from the soils of three wetland pine plantations, among which 63 were Fusarium, two were Alternaria and one was Rhizoctonia fungi. Pathogenicity tests on 66 fungal strains of pine seedling damping-off disease showed that 12 fungal strains were pathogenic or weak pathogenic fungi, 17 Fusarium strains were strongly pathogenic fungi and the remaining 37 were Moderately pathogenic fungi. A total of 320 strains of bacteria were isolated from the roots of P. elliottii. The plate antagonistic assay showed that 10 of these strains had a strong antagonistic effect on the pathogen fungi of pine seedling damping-off disease and had greater potential for biocontrol against the damping-off disease of pine seedlings. The 16S rDNA sequence analysis showed that the taxonomic status of these 10 biocontrol strains were: Burkholderia cepacia 7, Bacillus subtilis 2 and Bacillus subtilis 1.