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目的探讨原花青素对大肠癌HT-29细胞增殖及Pim-1、Bcl-2、CyclinD1基因的影响。方法取对数生长期HT-29细胞,分别加入100 mg/L、200 mg/L、400 mg/L、800 mg/L原花青素培养,采用四甲基偶氮唑蓝(MTT)法检测细胞增殖;采用荧光定量PCR检测Pim-1、Bcl-2、CyclinD1 mRNA表达。结果 24 h时,各浓度组原花青素对HT-29细胞增殖抑制率分别为(4.28±0.34)%、(10.05±0.61)%、(16.67±0.63)%、(28.16±0.91)%;48 h时,各浓度组原花青素对HT-29细胞增殖抑制率分别为(11.47±1.53)%、(17.65±1.21)%、(30.82±1.99)%、(44.48±2.18)%,对Pim-1、Bcl-2、CyclinD1mRNA表达有抑制作用(P<0.05),且作用呈浓度依赖性。结论原花青素体外可呈浓度依赖性抑制HT-29细胞增殖,其机制可能与抑制Pim-1、Bcl-2、CyclinD1表达有关。
Objective To investigate the effects of proanthocyanidins on the proliferation and the expression of Pim-1, Bcl-2 and CyclinD1 genes in HT-29 cells. Methods HT-29 cells were treated with 100 mg / L, 200 mg / L, 400 mg / L and 800 mg / L proanthocyanidins respectively. Cell proliferation was measured by MTT assay. The mRNA expression of Pim-1, Bcl-2 and CyclinD1 were detected by real-time PCR. Results The inhibitory rates of proanthocyanidins on the proliferation of HT-29 cells were (4.28 ± 0.34)%, (10.05 ± 0.61)%, (16.67 ± 0.63)%, (28.16 ± 0.91)% at 24 h The inhibitory rates of proanthocyanidins on the proliferation of HT-29 cells were (11.47 ± 1.53)%, (17.65 ± 1.21)%, (30.82 ± 1.99)% and (44.48 ± 2.18)%, respectively. 2, CyclinD1mRNA expression was inhibited (P <0.05), and the effect was concentration-dependent. Conclusion Proanthocyanidins inhibit the proliferation of HT-29 cells in a concentration-dependent manner in vitro, which may be related to the inhibition of the expression of Pim-1, Bcl-2 and CyclinD1.