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目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)对人神经母细胞瘤的作用及干扰素-γ(IFN-γ)对新型凋亡分子TRAIL抗瘤活性的影响,并探讨其影响机制。方法应用MTT分析和流式细胞仪研究TRAIL对人神经母细胞瘤细胞的抑制作用及IFN-γ对TRAIL抗瘤活性的影响。用RT-PCR方法检测IFN-γ作用48h后,Caspase-8-mRNA的表达。结果TRAIL(10,50,100μg/L)单独作用、IFN-γ(1000U/mL)单独作用后,对SY5Y细胞的增殖抑制率分别为(4·38±0·89)%、(3·54±1·66)%、(5·03±1·26)%、(5·33±2·06)%;凋亡率分別为(5·18±3·33)%、(5·26±3·64)%、(5·00±2·88)%、(8·14±7·35)%。IFN-γ(1000U/mL)与TRAIL(100μg/L)联合作用后,对SY5Y细胞的增殖抑制率为(41·22±7·09)%;凋亡率为(21·29±6·20)%。RT-PCR方法发现IFN-γ作用48h后Caspase-8-mRNA的表达明显上调。结论神经母细胞瘤细胞SH-SY5Y对TRAIL不敏感,IFN-γ可以明显提高TRAIL对神经母细胞瘤细胞的敏感性,其发生机制可能是通过IFN-γ上调Caspase-8-mRNA的表达而实现的。
Objective To investigate the effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on human neuroblastoma and the effect of interferon-γ (IFN-γ) on the anti-tumor activity of TRAIL. Methods The inhibitory effect of TRAIL on human neuroblastoma cells and the effect of IFN-γ on the anti-tumor activity of TRAIL were studied by MTT assay and flow cytometry. The expression of Caspase-8-mRNA was detected by RT-PCR 48 h after IFN-γ treatment. Results The inhibitory rates of TRAIL (10,50,100μg / L) alone and IFN-γ (1000U / mL) alone on SY5Y cells were (4.38 ± 0.89)%, (3.54 ± The percentages of apoptosis were (5.18 ± 3.33)%, (5.26 ± 3)%, (5.33 ± 1.26)%, (5.33 ± 2.06)% respectively · 64%, (5 · 00 ± 2 · 88)%, (8 · 14 ± 7 · 35)%. The synergistic effect of IFN-γ (1000U / mL) and TRAIL (100μg / L) on the proliferation of SY5Y cells was (41.22 ± 7.90)%, and the apoptosis rate was (21.29 ± 6.20) )%. The expression of Caspase-8-mRNA was up-regulated after IFN-γ treatment for 48h by RT-PCR. Conclusion SH-SY5Y neuroblastoma cells are not sensitive to TRAIL, IFN-γ can significantly increase the TRAIL sensitivity of neuroblastoma cells, its mechanism may be through IFN-γ upregulation of Caspase-8-mRNA expression of.