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目的:测定不同运动负荷时大鼠股外肌组织中一氧化氮-一氧化氮合酶(NO-NOS)体系的各成分含量或活性的变化,探讨运动训练对股外肌的影响。方法:将60只雄性SD大鼠随机分为3组,即安静对照组、中等运动强度组、运动疲劳组,每组20只。对安静对照组大鼠进行常规饲养,不加任何干预,对中等运动强度组大鼠进行中等强度游泳训练,对运动疲劳组大鼠建立运动性疲劳模型。采用生化试剂盒测定各组大鼠末次训练后股外肌组织NO-NOS体系含量或活性。结果:与安静对照组比较,中等运动强度组大鼠股外肌组织中NO含量、总一氧化氮合酶(tNOS)、内皮型一氧化氮合酶(eNOS)、神经元型一氧化氮合酶(nNOS)、诱导型一氧化氮合酶(iNOS)活性水平均显著升高(P<0.05或P<0.01);运动疲劳组大鼠的股外肌组织NO含量,tNOS,eNOS,nNOS活性水平均无显著变化,其中NO含量,tNOS,nNOS活性水平有升高趋势。与中等运动强度组比较,运动疲劳组大鼠iNOS活性水平显著降低(P<0.05)。结论:股外肌组织中NO-NOS体系各成分不同运动负荷作用下会产生不同程度的变化,中等强度运动可导致股外肌组织中的NO含量和tNOS,eNOS,nNOS,iNOS活性的升高,而疲劳运动则有引起iNOS,eNOS活性降低的趁势。
OBJECTIVE: To determine the changes of the content or activity of NO-NOS in rat’s extrasomal muscle during different exercise load, and to explore the effect of exercise training on the extra-femoral muscle. Methods: Sixty male Sprague-Dawley rats were randomly divided into three groups: control group, moderate exercise intensity group and exercise-induced fatigue group, with 20 rats in each group. Rats in the quiet control group were routinely housed, without any intervention, moderate-intensity swimming training was performed on rats of moderate exercise intensity, and exercise-induced fatigue model was established in rats of exercise-induced fatigue. Biochemical kits were used to determine the content or activity of NO-NOS in the extra-muscular tissues of the rats after the last training. Results: Compared with resting control group, NO content, total nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS), neuronal nitric oxide synthase The levels of nNOS and iNOS were significantly increased (P <0.05 or P <0.01). NO content, tNOS, eNOS and nNOS activity No significant changes in the level of NO, tNOS, nNOS activity levels have increased. Compared with the moderate exercise intensity group, the iNOS activity of the exercise-induced fatigue group was significantly lower (P <0.05). CONCLUSIONS: The NO-NOS system in different parts of the femoral muscle can change to varying degrees under different exercise load. Moderate-intensity exercise can lead to the increase of NO content, tNOS, eNOS, nNOS and iNOS activity , While fatigue exercise has caused iNOS, eNOS activity decreased.