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目的研究骨髓间充质干细胞(BMSCs)脑内移植后对缺血性脑卒中大鼠的行为学影响。方法采集志愿者骨髓,分离、培养BMSCs;建立大鼠大脑中动脉栓塞缺血2h再灌注模型,分为治疗组12只和对照组12只,24h后脑内注射hBMSCs悬液15μL(2×1010L-1)和D-hank’s液15μL;移植后第4天开始进行NSS评分、黏贴物移除实验、转棒实验及Morris水迷宫实验的循环测试。结果治疗后第8天,水迷宫平均逃逸时间治疗组显著短于对照组(P<0.05),第29天趋同;平均游泳路程治疗组显著少于对照组,第32天趋同。第10天后,转棒实验(10r/min)平均潜伏期治疗组显著高于对照组。第13天,黏贴物移除实验平均潜伏期细胞治疗组显著快于对照组。NSS评分仅两个时间点有统计学差异。结论BMSCs脑内移植可以在卒中早期显著促进大鼠学习记忆、运动及感觉神经功能的恢复。
Objective To investigate the behavioral effects of bone marrow mesenchymal stem cells (BMSCs) transplantation in ischemic stroke rats after intracerebral transplantation. Methods The BMSCs were isolated and cultured in vitro. The model of ischemia-reperfusion in middle cerebral artery occlusion rats was established 2 hours after reperfusion. The rats in treatment group (12 rats) and control group (12 rats) were injected intraperitoneally with 15μL hBMSCs suspension (2 × 1010L- 1) and D-hank’s solution 15 μL; on the 4th day after transplantation, the NSS score, the adhesive removal test, the rotarod test and the Morris water maze test were performed. Results On the 8th day after treatment, the average escape time of the water maze was significantly shorter in the treatment group than in the control group (P <0.05), and was similar on the 29th day. The average swimming distance was significantly less in the treatment group than in the control group, and was similar on the 32nd day. After 10 days, the mean incubation period in the rotarod test (10r / min) group was significantly higher than that in the control group. On the 13th day, the average latency of the adhesive removal experimental group was significantly faster than the control group. NSS score was only statistically significant at two time points. Conclusion Transplantation of BMSCs intracerebral can significantly promote learning and memory, motor and sensory nerve function recovery in early stroke.