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目的探讨胎盘组织中胰岛素样生长因子Ⅱ(IGF-Ⅱ)在子痫前期发病中的作用及意义。方法采用RT-PCR和Western-Blot方法检测30例子痫前期患者(其中轻度20例,重度10例,子痫前期组)和15例正常同期妊娠产妇(对照组)胎盘中IGF-ⅡmRNA及其蛋白的表达。结果(1)子痫前期组胎盘中IGF-ⅡmRNA的表达水平为0.74±0.42,显著低于对照组的0.97±0.12(P<0.01)。(2)子痫前期组胎盘中IGF-II蛋白表达水平为0.65±0.96,显著低于对照组的0.82±0.35(P<0.01)。结论子痫前期患者胎盘组织中IGF-II水平降低,可能在该病的发病中起重要作用。
Objective To investigate the role and significance of insulin-like growth factor-Ⅱ (IGF-Ⅱ) in the pathogenesis of preeclampsia in placenta. Methods RT-PCR and Western-blot were used to detect the expression of IGF-ⅡmRNA in placenta of 30 preeclampsia patients (20 mild cases, 10 severe cases, preeclampsia group) and 15 normal pregnant women (control group) Protein expression. Results (1) The expression of IGF-ⅡmRNA in placenta of preeclampsia group was 0.74 ± 0.42, significantly lower than that of the control group (0.97 ± 0.12, P <0.01). (2) The level of IGF-II protein in placenta of preeclampsia group was 0.65 ± 0.96, which was significantly lower than that of the control group (0.82 ± 0.35, P <0.01). Conclusions The decrease of IGF-II in placenta of preeclampsia may play an important role in the pathogenesis of this disease.