论文部分内容阅读
为找到快速而有效地测定紫花苜蓿(Medicago sativa L.)花粉生活力的方法,本文通过碘-碘化钾染色、氯化三苯基四氮唑(TTC)染色、红墨水染色以及花粉离体培养萌发4种方法测定紫花苜蓿花粉的生活力。结果表明:前3种染色的方法不能准确快速的测定肇东苜蓿花粉生活力,离体培养萌发法中的琼脂法和悬滴法可以快速而准确的测定肇东苜蓿花粉的生活力。琼脂法和悬滴法培养花粉1.5h其萌发率最高,平均萌发率为55%,但悬滴法更加快捷方便,萌发率是54%,最适宜的浓度为蔗糖10%,硼酸0.015%。
In order to find a rapid and effective method for the determination of the pollen viability of alfalfa (Medicago sativa L.), Iodine-potassium iodide staining, triphenyltetrazolium chloride (TTC) staining, red ink staining and in vitro pollen germination Four ways to determine the viability of alfalfa pollen. The results showed that the former three methods could not determine the pollen viability of alfalfa alfalfa quickly and accurately. The agar and hanging drop method of in vitro germination method could rapidly and accurately determine the pollen viability of Zhaodong alfalfa. The germination rate was the highest when the pollen was cultured by agar method and hanging drop method for 1.5h. The average germination rate was 55%. However, the hanging drop method was faster and more convenient. The germination rate was 54%. The most suitable concentration was 10% sucrose and 0.015% boric acid.