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应用基质辅助红外激光解吸离子化/博里叶变换质谱法(IR-MALDI/FTMS),对九肽药物促黄体素释放激素类似物(LRH-A)的分子量及其氨基酸序列进行测定。用2,5-二羟基苯甲酸(DHB)作为基质,测得准分子离子(M+H)~1的m/z=1167.61006,与理论计算值的相对误差为3.66ppm。调节激光能量,获得一系列典型的碎片离子,从而确证了LRH-A的氨基酸序列。此法不需对样品进行预处理。
The molecular weight and amino acid sequence of the nine-peptide drug luteinizing hormone releasing hormone analogue (LRH-A) were determined by matrix-assisted infrared laser desorption / ionization / Fourier transform mass spectrometry (IR-MALDI / FTMS) Using 2,5-dihydroxybenzoic acid (DHB) as a substrate, the m / z = 1167.61006 of the quasi-molecular ion (M + H) ~ 1 was measured and the relative error from the theoretical value was 3.66ppm. The laser energy was adjusted to obtain a series of typical fragment ions, confirming the amino acid sequence of LRH-A. This method does not require sample pretreatment.