以壳聚糖为载体,戊二醛作为交联剂,采用载体交联法制备固定化木瓜蛋白酶,并研究了固定化木瓜蛋白酶的最佳固定化条件。结果表明:木瓜蛋白酶的最佳固定化条件为给酶量为40～50mg/g,于pH7.5,25～30℃下,0.4%～0.5%的戊二醛溶液交联12h,所得的固定化木瓜蛋白酶的活力回收率平均达61.6%。 Chitosan was used as carrier, glutaraldehyde was used as cross-linking agent, and immobilized papain was prepared by carrier cross-linking method. The optimal immobilization conditions of immobilized papain were studied. The results showed that the optimal immobilization conditions for papain were as follows: the amount of enzyme was 40 ~ 50mg / g, and the solution of glutaraldehyde 0.4% ~ 0.5% was crosslinked at pH 7.5,25 ~ 30 ℃ for 12 h. The vitality recovery of papain averaged 61.6%.