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本文介绍用伊文思蓝法观察呋喃丙胺对小白鼠胃的刺激作用。动物于口服药物后即由尾静脉注入1％伊文思蓝,并于2小时后剖杀取胃,根据胃底浆膜面的蓝色色素沉着情况,评价药物对胃的刺激作用。试验证明,小白鼠一次口服呋喃丙胺或二甲苯后,用伊文思蓝法观察药物对胃的刺激作用与组织病理学的观察结果相仿。这种方法具有操作简便、快速、灵敏和重现性高等优点。在拮抗试验中,发现扑尔敏、异丙嗪、苯海拉明和氯丙嗪具有较强的拮抗呋喃丙胺对鼠胃的刺激作用,抗炎类药物次之,而阿托品则甚差。用伊文思蓝法观察的结果表明,小白鼠每天口服呋喃丙胺500 mg/kg,连续10天时,药物对鼠胃的刺激作用逐渐减轻,至给药10天后,胃的刺激反应仅及第1次给药时的1/3。若在疗程的第1～3天,每天于口服呋喃丙胺前1小时先服扑尔敏,则呋喃丙胺对鼠胃的刺激作用,仅在给药的第三天较明显,但轻于单服呋喃丙胺3天组。用肉眼或组织病理学方法观察胃肠病变的结果,与伊文思蓝法的基本一致。扑尔敏不影响呋喃丙胺的抗血吸虫作用。
This article describes the use of Evans blue method of furosemide to stimulate the stomach of mice. The animal was injected with 1% Evans Blue via the tail vein after oral administration, and the stomach was dissected 2 hours later. According to the blue pigmentation in the fundic serous surface, the stimulus to the stomach was evaluated. Experiments show that a mouse oral administration of furosemide or xylene, with Evans blue method to observe the drug’s stomach stimulation and histopathological observations. This method is simple, fast, sensitive and reproducible. In the antagonistic test, it was found that chlorpheniramine, promethazine, diphenhydramine and chlorpromazine had strong antagonistic effects on furosemide in rat stomach, followed by anti-inflammatory drugs, while atropine was very poor. Evans Blue method observed the results show that mice daily oral administration of furosemide 500 mg / kg for 10 days, the drug gradually reduce the stimulation of the rat stomach, to the administration of 10 days after the stomach stimulation reaction only the first 1/3 when administered. If in the course of treatment 1 to 3 days, daily oral administration of furosemide 1 hour before taking chlorpheniramine, the furosemide on rat stomach stimulation, only the third day of administration more obvious, but lighter than the single service Furan propylamine 3-day group. Observed with the naked eye or histopathological results of gastrointestinal lesions, and Evans blue method is basically the same. Chlorpheniramine does not affect the anti-schistosome effect of furosemide.