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目的探讨不同缺氧损伤细胞模型下黑青稞籽皮类黄酮物质对缺氧损伤细胞的保护作用。方法采用柠檬酸-乙醇浸提法富集黑青稞籽皮类黄酮物质,并对类黄酮含量进行两种不同方法检测和定量,用三种不同的细胞模型验证其对缺氧损伤的保护作用。结果实验条件下得到黑青稞籽皮提取物30.3 mg/g,通过光谱分析及HPLC检测发现,籽皮中的类黄酮物质多为花青素类,花青素提取率为68.6 mg/g,其中氯化花翠素占提取物的57 mg/g;以芦丁标定类黄酮总量达到69.4 mg/g;实验所用剂量条件下,黑青稞籽皮类黄酮表现出了对三种缺氧损伤细胞的保护作用,并能有效清除活性氧自由基,提高过氧化物歧化酶的活性。结论黑青稞籽皮提取物发挥缺氧损伤保护作用的物质基础可能是花青素类黄酮,它对防护活性氧介导的细胞损伤具有重要意义。
Objective To investigate the protective effects of flavonoids from seed hulls of Hippophae rhamnoides on hypoxic injury cells under different hypoxic injury cell models. Methods Citric acid - ethanol extraction was used to enrich the flavonoids of the seed coat of Atractylodes macrocephala. The flavonoid content was detected and quantified by two different methods. Three different cell models were used to verify its protective effect against hypoxia injury. Results The results showed that 30.3 mg / g of the extract of black sesame seed extract was obtained under the experimental conditions. The results of spectral analysis and HPLC showed that most of the flavonoids in the seed coat were anthocyanidins with the anthocyanin extraction rate of 68.6 mg / g Chlorpheniramine accounted for 57 mg / g of the extract; the total amount of flavonoids was determined to be 69.4 mg / g with rutin; under the conditions of the experiment, The protective effect, and can effectively remove reactive oxygen free radicals and improve the activity of superoxide dismutase. Conclusion The material basis of the protective effect of the extract of black sesame seed on hypoxia may be anthocyanin flavonoids, which are important for the protection of reactive oxygen species against cell injury.