【摘 要】
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Objective: To observe the differential expression of TGF-β-induced gene human clone 3 (βig-h3) in human hepatoma cell lines. Methods: Human hepatoma cells HHC
【出 处】
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中国人民解放军军医大学学报（英文版）
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Objective: To observe the differential expression of TGF-β-induced gene human clone 3 (βig-h3) in human hepatoma cell lines. Methods: Human hepatoma cells HHCC, 7721, T7721 constructed by stably transfecting HAb18G/CD147 cDNA into 7721 and normal human liver cell QZG were cultured as previously. RT-PCR and western blot were used to investigate the differential expression of βig-h3 in hu man hepatoma cell lines and normal liver cell. Results: The results of RT-PCR suggested that the expres sion of βig-h3 mRNA in human hepatoma cells was higher than that in normal human liver cell QZG (P＜0.01), and its expression level in human hepatoma cells in turn was HHCC＞T7721＞7721. Moreover,the similar results of βig-h3 protein expression were testified by western blot. Conclusion: This study demonstrates that the expression of βig-h3 in hepatoma cell lines is higher than that in normal liver cell QZG, which provides a sound basis for exploring the function of βig-h3 in processes of adhesion and metas tasis of human hepatoma cells.
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