目的:研究多巴胺(DA)诱发神经元的电流反应。 方法:制霉菌素打孔的膜片箝全细胞记录。 结果:在-20mV箝制电压下,DA(0.1—1mmol·L~(-1))对26％黑质神经元(5/19个)引起外向电流;对36％海马CA_1锥体细胞(25/69个)出现3种反应:外向电流伴有膜电导增加、缓慢内向电流伴随膜电导减小、外向—内向电流。DA引起的CA_1锥体细胞电流反应的阈剂量为3mmol·L~(-1),无电压依赖关系。翻转电位(E_(DA))接近K~+平衡电位,为TEA抑制。结论:DA诱发海马CA_1锥体细胞的外向电流可能是K~+电流。 Objective: To study the current response of dopamine (DA) -induced neurons. Methods: Whole-cell patch-clamp recording of nystatin-punctured cells. RESULTS: DA (0.1-1 mmol·L -1) induced outward currents in 26% of substantia nigra neurons (5/19) at a -20 mV clamping voltage. In 36% of hippocampal CA 1 pyramidal cells (25 / 69) appeared three kinds of reactions: outward current accompanied by increased membrane conductivity, slow inward current with the membrane conductivity decreases, outward - inward current. The threshold dose of DA induced CA 1 pyramidal cell current response was 3 mmol·L -1, with no voltage dependence. Turnover potential (E_ (DA)) close to K ~ + balance potential, for the TEA inhibition. Conclusion: The outward current induced by DA in CA1 pyramidal cells of hippocampus may be K ~ + current.