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采用放射配体结合实验，离体左心房收缩功能实验，逆转录聚合酶链反应及高效液相色谱等方法研究长期饮用普萘洛尔（７０ｍｇｋｇ－１ｄ－１，８ｗｋ）对大鼠心脏β肾上腺素受体（β－ＡＲ）及其亚型，外周血淋巴细胞β２－ＡＲ和血浆儿茶酚胺水平的影响．结果表明长期饮用普萘洛尔后：（１）心脏β－ＡＲ密度从对照组的４１±８ｐｍｏｌｇ－１蛋白上调到６１±９ｐｍｏｌｇ－１蛋白，ＣＧＰ２０７１２Ａ竞争抑制曲线２位点分析结果显示为β１和β２－ＡＲ同等程度上调；（２）心脏β１－ＡＲｍＲ－ＮＡ水平不变而β２－ＡＲｍＲＮＡ水平显著增加；（３）β－ＡＲ及其亚型介导的离体左心房正性变力效应增强，以β２－ＡＲ的功能改变更为显著；（４）血淋巴细胞β２－ＡＲ数目从对照组的１９±７ｐｍｏｌｇ－１蛋白上调至３２±８ｐｍｏｌｇ－１蛋白；（５）血浆去甲肾上腺素水平（０．２１±０．１２μｇＬ－１）明显低于对照组（０．３８±０．１５μｇＬ－１）．
Radioligand binding assay, isolated left atrial systolic function test, reverse transcription polymerase chain reaction and high performance liquid chromatography were used to study the effects of long-term drinking propranolol (70mgkg-1d-1,8wk) on β adrenal (Β-AR) and its subtypes, peripheral blood lymphocytes β2-AR and plasma catecholamine levels. The results showed that after prolonged drinking of propranolol: (1) the density of cardiac β-AR was increased from 41 ± 8 pmolg-1 protein in the control group to 61 ± 9 pmolg-1 protein, and the results of competition inhibition curve 2 of CGP20712A showed that β1 and β2 -AR increased to the same extent; (2) the level of cardiac β1-ARmR-NA was unchanged and the level of β2-AR mRNA was significantly increased; (3) The positive effect of left atrium mediated by β-AR and its subtype was enhanced, (4) The number of β2-AR in lymphocytes increased from 19 ± 7 pmolg-1 protein in the control group to 32 ± 8 pmolg-1 protein; (5) Plasma norepinephrine level 0.21 ± 0.12μgL-1) was significantly lower than the control group (0.38 ± 0.15μgL-1).