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速激肽族肽,包括K物质、P物质、神经素B等都具有神经递质的功能,它们越来越引起人们的关注。免疫学方法诸如:放射免疫测定法、免疫组织化学法等对神经肽的分布、定位和生物学功能等方面的研究提供了极大的方便。我们以K物质-甲状腺球蛋白偶联物免疫动物——Wistar大鼠和Balb/c小鼠。Wistar大鼠对K物质-甲状腺球蛋白的免疫应答能力显著高于Balb/c小鼠。通过Wistar大鼠脾细胞与小鼠骨髓瘤细胞进行异种细胞融合,获得了一株稳定地分泌抗K物质的单克隆抗体的细胞系(SE-1)。用RIA检测该单克隆抗体的特异性,除与P物质有一些交叉外,与其余我们所用的脑肽均不反应。
Tachykinins peptides, including substance K, substance P and neurotrophin B, all have the function of neurotransmitters. They are attracting more and more attention. Immunological methods such as: radioimmunoassay, immunohistochemical methods such as the distribution of neuropeptides, localization and biological functions and other aspects of the study provides a great convenience. We immunized animals with K substance-thyroglobulin conjugates - Wistar rats and Balb / c mice. Wistar rats on the K substance - thyroglobulin immune response was significantly higher than Balb / c mice. A cell line (SE-1) stably secreting anti-K monoclonal antibody was obtained by xenogeneic cell fusion of Wistar rat spleen cells and mouse myeloma cells. The specificity of this monoclonal antibody detected by RIA, except for some crossover with substance P, did not react with the rest of the brain peptides we used.