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在0.01~100MHz频率内,使用Agilent 4294A阻抗分析仪测量了人肝癌SMMC-7721细胞的交流阻抗,通过电阻抗谱、Bode图、Nyquist图和Nichols图观察了细胞体积分数(CVF)对肝癌细胞电阻抗特性的影响。结果表明:1频率依赖性:电阻抗实部增量、虚部增量(ΔZ′、ΔZ″)、幅模增量(Δ|Z*|)和相位角增量(Δθ)皆随频率发生变化;2CVF依赖性:当CVF增加时,低频极限增量值(ΔZ′0、Δ|Z*|0)、峰值(ΔZ″p、Δθp)、曲线面积和半径(Nyquist图、Nichols图)均随之增大;3存在两个特征频率:第一特征频率(fC1)和第二特征频率(fC2),分别来源于细胞膜与细胞外液、细胞质交界面的极化作用。结论:电阻抗谱方法能够观察人肝癌细胞电特性,可用于探讨肝癌细胞电生理机制变化,为进一步筛选抗肿瘤药物提供研究手段和观测指标,具有重要的理论价值和潜在的应用前景。
The AC impedance of human hepatocellular carcinoma SMMC-7721 cells was measured by Agilent 4294A impedance analyzer in the frequency of 0.01 ~ 100MHz. The effects of cell volume fraction (CVF) on the resistance of hepatoma cells were investigated by electrical impedance spectroscopy, Bode plot, Nyquist plot and Nichols plot. Anti-characteristic effects. The results show that: 1 frequency dependence: the real part of the electrical impedance increments, the imaginary part increment (ΔZ ', ΔZ “), the amplitude increment (Δ | Z * |) and the phase angle increment (Δθ) 2CVF Dependency: As the CVF increases, both the low-frequency limit increments (ΔZ'0, Δ | Z * | 0), peak (ΔZ ”p, Δθp), curve area and radius (Nyquist plot, Nichols plot) (FC1) and the second characteristic frequency (fC2), which are derived from the polarization of the interface between cell membrane and extracellular fluid and cytoplasm, respectively. CONCLUSION: Electrical impedance spectroscopy can observe the electrical characteristics of human hepatocellular carcinoma cells, and can be used to investigate the changes of electrophysiological mechanism of hepatocellular carcinoma cells. It provides an important research tool and observation index for screening anti-tumor drugs. It has important theoretical value and potential application prospects.