论文部分内容阅读
目的 :探讨天佛参口服液诱导人喉癌 Hep- 2细胞凋亡及其机制。方法 :采用体外细胞培养实验方法。应用 MTT、透射电镜、DNA琼脂糖凝胶电泳、流式细胞仪检测、原位末端标记等技术 ,观察药物对细胞的抑制率及凋亡形态 ,检测凋亡率及胞内 [Ca2 +]变化。结果 :MTT测得细胞抑制率随时间、剂量而增大 ,透射电镜下见到凋亡细胞及凋亡小体形成 ,DNA琼脂糖凝胶电泳结果显示清晰的 DNA梯状条带 ,原位末端标记检测出细胞凋亡率亦有时间、剂量依赖性 ,细胞内 [Ca2 +]随药物浓度的增加而升高 ,且具有统计学意义。结论 :天佛参口服液确有诱导 Hep- 2细胞凋亡的作用 ,其机制可能与胞内 [Ca2 +]变化有关。
Objective: To investigate the mechanism of Tianfo Shen oral liquid in inducing apoptosis of human laryngeal carcinoma Hep-2 cells. Methods: The in vitro cell culture experiment method was used. The inhibitory rate and apoptotic morphology of the cells were observed by MTT, transmission electron microscopy, DNA agarose gel electrophoresis, flow cytometry and in situ end labeling. The apoptosis rate and intracellular [Ca2 +] change were observed . Results: The inhibitory rate of MTT increased with time and dose, and apoptotic cells and apoptotic bodies were observed under transmission electron microscope. DNA ladder agarose gel electrophoresis showed clear DNA ladder, The rate of apoptotic cell apoptosis was also marked by time and dose-dependent. The intracellular [Ca2 +] increased with the increase of the drug concentration, which was statistically significant. Conclusion: Tianfo Shen oral liquid does induce apoptosis of Hep-2 cells, and its mechanism may be related to intracellular [Ca2 +] changes.