目的:探讨脑血管内皮细胞的体外培养方法并进行形态观察;方法:采用新生Wistar大鼠,通过匀浆、两次过滤法收集大鼠脑微血管段;将经胶原酶处理后的脑微血管段静置培养5～7天后,即可分离培养出纯度较高的脑微血管内皮细胞;结果:培养细胞形态呈长梭形与多角形,多数有边缘突起,电镜下可见细胞间有紧密连接结构.经Ⅷ因子相关抗原免疫组化检测为阳性.结论:通过匀浆、两次过滤法收集的大鼠脑微血管段中能生长出脑微血管内皮细胞. OBJECTIVE: To investigate the culture method of cerebrovascular endothelial cells in vitro and to observe the morphological changes. Methods: Newborn Wistar rats were used to collect brain microvascular segments by homogenate and twice filtration. After culturing for 5-7 days, the brain microvascular endothelial cells with high purity could be isolated and cultured. Results: The cultured cells were fusiform and polygonal in shape, most of them had edge protrusions, and the cells were closely connected by electron microscopy. Immunohistochemical detection of factor-related antigen was positive.Conclusion: Cerebral microvascular endothelial cells can grow in the brain microvessel collected from rats by homogenization and twice filtration.