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目的建立桂参通脉胶囊质量标准。方法采用薄层色谱鉴别对桂参通脉胶囊处方中葛根、郁金及茯苓进行定性鉴别;采用高效液相色谱(HPLC)法对桂枝中桂皮醛进行含量测定。色谱柱:C18色谱柱(4.6 mm×150 mm,5μm);流动相:乙腈—水(32∶68);检测波长:290 nm。结果在薄层色谱中检出葛根、郁金及茯苓;桂皮醛在0.020 5~0.205 0μg(r=0.999 8)范围内呈现良好的线性关系,平均回收率为98.6%,标准偏差(RSD)为0.68%(n=6)。结论该方法准确、可靠,可用于桂参通脉胶囊的质量控制。
Objective To establish the quality standard of Guishen Tongmai capsule. Methods The identification of Pueraria lobata, Turmeric and Poria cocos were conducted by TLC. The content of cinnamic aldehyde in Guizhi was determined by high performance liquid chromatography (HPLC). Column: C18 column (4.6 mm × 150 mm, 5 μm); mobile phase: acetonitrile-water (32:68); detection wavelength: 290 nm. Results Pueraria, turmeric and Poria cocos were detected by TLC. Cinnamic aldehyde showed a good linear relationship in the range of 0.020 5-0.205 0 μg (r = 0.999 8) with the average recovery of 98.6% and the standard deviation (RSD) 0.68% (n = 6). Conclusion The method is accurate and reliable and can be used for the quality control of Guishen Tongmai Capsules.