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目的:研究猪牙皂正丁醇提取物体外对肝星状细胞活性的影响,为肝纤维化的防治提供参考。方法:将肝星状细胞株(HSC-T6)细胞以猪牙皂正丁醇提取物刺激24 h,采用MTT法检测其增殖情况;采用流式细胞仪检测其凋亡情况;采用Western Blot检测其Ⅰ型胶原蛋白的表达水平。结果:猪牙皂正丁醇提取物体外可抑制HSCT6细胞增殖并促进其凋亡,还可下调Ⅰ型胶原蛋白的表达水平。结论:体外实验证实猪牙皂正丁醇提取物具有抑制肝星状细胞活性的作用。
Objective: To study the effect of n-butanol extract of porcine tooth soap on the activity of hepatic stellate cells in vitro and provide references for the prevention and treatment of hepatic fibrosis. Methods: Hepatic stellate cell line (HSC-T6) cells were stimulated with n-butanol extracts of porcine tooth soap for 24 h. The proliferation of HSC-T6 cells was detected by MTT assay. The apoptosis was detected by flow cytometry. Type Ⅰ collagen expression levels. Results: Butanol extract of porcine toothpaste could inhibit the proliferation and promote the apoptosis of HSCT6 cells in vitro, and also down-regulate the expression of type Ⅰ collagen. Conclusion: In vitro experiments confirmed that n-butanol extract of pork toothpaste has the effect of inhibiting hepatic stellate cell activity.