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目的探讨疑似深部真菌感染患者血中(1→3)-β-D-葡聚糖的浓度与感染的关系。方法采用日本生化学工业株式会社的(1→3)-β-D-葡聚糖测定G-testTE试剂盒检测了13例患者血浆中的(1→3)-β-D-葡聚糖的含量,以UV-2450紫外可见光分光光度仪检测波长545nm的吸光度值,根据特定检测浓度换算公式得出标本的(1→3)-β-D-葡聚糖含量。结果13例被检测患者中9例经培养证实为深部真菌感染者血浆的β-D-葡聚糖含量皆较高,最高者可达352.94pg/mL(为真菌混合感染患者),平均可达203.47pg/mL;4例真菌培养阴性患者血浆β-D-葡聚糖水平,3例超过54.40pg/mL,1例为16.16pg/mL。所有标本采用UV-2450紫外可见光分光光度仪在波长545nm检测,皆可出现较明显的吸收峰。采用GtestTE试剂盒检测13例疑似深部真菌感染患者血浆的β-D-葡聚糖含量,分析诊断深部真菌感染的有效性,结果其敏感度为92.31%,特异度100%,阳性预测值100%,阴性预测值为98.36%。结论GtestTE方法简便,反应快速,可用于真菌感染的早期诊断。
Objective To investigate the relationship between blood (1 → 3) -β-D-glucan concentration and infection in patients with suspected deep fungal infection. Methods The plasma levels of (1 → 3) -β-D-glucan in 13 patients were measured by the G-testTE kit (1 → 3) -β-D-glucan in Japan Biochemical Industries, UV-2450 UV-visible spectrophotometer was used to detect the absorbance at 545nm, and the content of (1 → 3) -β-D-glucan in the sample was obtained according to the formula of specific detection concentration. Results The plasma β-D-glucan contents in 9 of the 13 patients tested were confirmed to be deep fungal infections, with the highest being up to 352.94pg / mL (for fungal mixed infection), with an average of up to 203.47pg / mL. The plasma levels of β-D-glucan in 4 patients with negative fungal culture were more than 54.40pg / mL in 3 cases and 16.16pg / mL in 1 case. All samples using UV-2450 UV-visible spectrophotometer at a wavelength of 545nm detection, can be more obvious absorption peak. The GtestTE kit was used to detect the plasma β-D-glucan in 13 patients with suspected deep fungal infection. The results showed that the sensitivity, specificity and specificity were 92.31%, 100% and 100%, respectively. , Negative predictive value of 98.36%. Conclusion GtestTE method is simple, rapid response, can be used for early diagnosis of fungal infection.