Seed dormancy,germination and storage behavior of Magnolia sinica,a plant species with extremely sma

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Magnolia sinica is one of the most endangered Magnoliaceae species in China.Seed biology information concerning its long-term ex situ conservation and utilization is insufficient.This study investigated dormancy status,germination requirements and storage behavior of M.sinica.Freshly matured seeds germinated to ca.86.5% at 25/15 ℃ but poorly at 30 ℃;GA3 and moist chilling promoted germination significantly at 20 ℃.Embryos grew at temperatures (alternating or constant) between 20 ℃ and 25 ℃,but not at 5 ℃ or 30 ℃.Our results indicate that M.sinica seeds possibly have non-deep simple mor-phophysiological dormancy (MPD).Seeds survived desiccation to 9.27%o and 4.85% moisture content (MC)as well as a further 6-month storage at-20 ℃ and in liquid nitrogen,including recovery in vitro as excised embryos.The established protocol ensured that at least 58% of seedlings were obtained after both cold storage and cryopreservation.These results indicate that both conventional seed banking and cryopreservation have potential as long-term ex situ conservation methods,although further optimized approaches are recommended for this critically endangered magnolia species.
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