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本文研究了正常和患癌小鼠血清在体外对癌细胞及骨髓细胞的抑制作用。结果表明,无论是正常小鼠血清或患癌小鼠血清对癌细胞的DNA生物合成的抑制作用最强,对RNA生物合成的抑制较弱,而对蛋白质的生物合成的抑制最小。上述二种血清对癌细胞DNA生物合成的抑制作用较大,而对骨髓细胞DNA生物合成几无抑制作用。此外,还观察到正常小鼠血清对骨髓RNA生物合成也无明显抑制作用。由此可见,血清的这种抑制作用在代谢上以对DNA的生物合成为最显著,在细胞种类上以对癌细胞为最强烈。 Boland R.P.等报道,正常人血清对小鼠肿瘤细胞有细胞毒性作用。Nobel E.P.等也报道过正常人血清能抑制肿瘤的活性。最近Edwin V.G.等报告,正常成人血清能抑制体外培养初代乳腺癌上皮细胞,但对非恶性乳腺组织,如纤维腺瘤等,并无抑制其生长的作用。现有实验证明,正常小鼠血清及患癌小鼠血清对艾氏腹水癌细胞的DNA生物合成有显著的抑制作用。血清中这种具有抑制癌细胞生长的活性物质,除能抑制癌细胞的DNA生物合成外,在代谢上还有哪些作用?是否对癌细胞的RNA和蛋白质的生物合成也有影响?对正常细胞是否也有影响?为此,本文用氚—胸腺嘧啶核苷(~3H-TdR)、氚—尿嘧啶核苷(~3H-UR)及氚—亮氨酸(~3H-Leu)分别进行有关实验,以观察其对艾氏腹水癌细胞抑制的主要代谢途径,并选用小鼠骨髓细胞作为正常细胞的代表,以观察其对不同细胞是否具有相对的选择性。
This study investigated the inhibitory effects of normal and cancerous mouse serum on cancer cells and bone marrow cells in vitro. The results showed that serum from either normal mouse or cancer-bearing mice had the strongest inhibitory effect on DNA biosynthesis of cancer cells, with weaker inhibition of RNA biosynthesis and minimal inhibition of protein biosynthesis. The above two kinds of serum have a greater inhibitory effect on the DNA biosynthesis of cancer cells, but have little effect on the DNA biosynthesis of bone marrow cells. In addition, normal mouse serum was also observed to have no significant inhibitory effect on bone marrow RNA biosynthesis. It can be seen that this inhibition of serum is most metabolically significant for the biosynthesis of DNA, and it is the most intense for cancer cells in cell types. Boland R. P. et al. reported that normal human serum had cytotoxic effects on mouse tumor cells. Nobel E.P. et al. also reported that normal human serum can inhibit tumor activity. Recently, Edwin V.G. et al. reported that normal adult serum can inhibit the growth of primary breast cancer epithelial cells in vitro, but it does not inhibit the growth of non-malignant breast tissues such as fibroadenoma. Existing experiments have shown that serum from normal mice and mice with cancer have significant inhibitory effects on DNA biosynthesis of Ehrlich ascites carcinoma cells. This active substance that inhibits the growth of cancer cells in the serum, in addition to inhibiting the DNA biosynthesis of cancer cells, what are the effects on metabolism, whether it affects the RNA and protein biosynthesis of cancer cells, and whether it is normal cells? Also, for this purpose, this experiment was performed using thymidine-thymidine (~3H-TdR), 氚-uridine (~3H-UR), and leucine-leucine (~3H-Leu), respectively. To observe its main metabolic pathway for Ehrlich ascites carcinoma cells, and use mouse bone marrow cells as a representative of normal cells to observe whether it is relatively selective to different cells.