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关于瓣膜间质细胞在瓣膜修复过程及组织瓣膜形成过程中的作用日益受到关注[1] ,因而瓣膜间质细胞培养作为研究的基本手段和技术将会被广泛采用。现介绍人心脏瓣膜间质细胞的两种培养方法。一、材料和方法1.试剂 :Ｄｕｌｂｅｃｃｏ最低基础培养基(ＤＭＥＭ ,Ｇｉｂｃｏ公司产品
About the role of valve interstitial cells in the process of valve repair and tissue valve formation is increasingly concerned [1], so the culture of valve stromal cells as the basic means of research and technology will be widely used. Now introduced two kinds of human heart valve stromal cells culture method. I. Materials and Methods 1. Reagents: Dulbecco’s Minimum Essential Medium (DMEM, Gibco’s product