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本文报告在腹腔镜检查时,用反射分光光度法对慢性肝脏病患者作肝血流动力学原位研究。通过腹部小切口将光导石英纤维束(直径6mm)放入,此纤维束由二股纤维组成,一股导人照明光,一股接受反射光。在腹腔镜直视下,使光导纤维与肝脏表面轻轻接触,并给予一入射光,通过带有微处理机的分光光度计,在不同波长下测定肝脏表面反射光的光谱。测定569-650nm波长之间的光密度差(ΔEr 569~650)估算肝组织血液的血红蛋白含量。从569、577和586nm波长下吸收光谱变化估算肝组织毛细血管中血氧饱和度。作者观察了42名病人,男性34人,女性8人,年龄25-60岁。根据临床与生化检验(血清转氨酶、碱性磷酸酶、白蛋白、总胆红素、γ-谷氨酰转肽酶、凝血酶原时
This paper reports the in-situ hepatic hemodynamic study of patients with chronic liver disease by reflection spectrophotometry at laparoscopy. The photoconductive quartz fiber bundle (6 mm in diameter) was inserted through a small incision in the abdomen. The fiber bundle consisted of two fibers, one of which led to illuminating light and one to receive reflected light. Under laparoscopic vision, the optical fiber is gently contacted with the surface of the liver and given an incident light. The spectrum of the reflected light on the surface of the liver is measured at different wavelengths by a spectrophotometer with a microprocessor. Determination of optical density difference between 569-650nm wavelength (ΔEr 569 ~ 650) to estimate the hemoglobin content of liver tissue blood. Estimation of oxygen saturation in liver capillaries from changes in absorption spectra at 569, 577 and 586 nm wavelengths. The authors looked at 42 patients, 34 males and 8 females, aged 25-60 years. According to clinical and biochemical tests (serum aminotransferase, alkaline phosphatase, albumin, total bilirubin, γ-glutamyl transpeptidase, prothrombin time