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目的探讨苯对骨髓单个核细胞增殖抑制率的影响,同时了解血清及氨磷汀能否作为保护因素减轻苯对细胞的影响。方法实验分四组:空白对照组、(0.25、3.5、20μmol/L)苯组、(0.25、3.5、20μmol/L)苯+(2、10、50μg/mL)氨磷汀组、(0.25、3.5、20μmol/L)苯+血清组,通过细胞增殖检测(CCK-8法)并计算骨髓单个核细胞增殖抑制率。结果高浓度苯组较低、中浓度苯组更能抑制细胞增殖(P<0.01);(3.5、0.25μmol/L)苯+血清组细胞增殖抑制率均较相对应浓度的苯组减少(P<0.05或P<0.01);但20μmol/L苯+血清组与苯组间细胞增殖抑制率差异无统计学意义(P>0.05)。(0.25、3.5、20μmol/L)苯+(50、10、2μg/ml)氨磷汀,细胞增殖抑制率与相应浓度苯组比较,结果均具有显著性差异(P<0.01或P<0.05)。结论细胞增殖抑制率随苯剂浓度的增加而增加,苯毒性存在剂量依赖效应;血清及氨磷汀均能减轻苯对细胞的增殖毒性,可能可以作为苯中毒细胞的保护剂。
Objective To investigate the effect of benzene on the inhibition of proliferation of bone marrow mononuclear cells and to find out whether serum and amifostine can reduce the effect of benzene on cells. Methods The experiment was divided into four groups: blank control group, benzene group (0.25, 3.5, 20μmol / L), amifostine group (0.25,10.5μmol / L) 3.5, 20μmol / L) benzene + serum group. The cell proliferation assay (CCK-8 method) and the inhibition rate of bone marrow mononuclear cell proliferation were calculated. Results The concentration of benzene in the high concentration benzene group was lower than that in the benzene concentration group (P <0.01). The inhibition rate of benzene in the group of (3.5,0.25μmol / L) <0.05 or P <0.01). However, there was no significant difference in the inhibition rate of cell proliferation between 20 μmol / L benzene + serum group and benzene group (P> 0.05). (0.25, 3.5, 20μmol / L) benzene + (50,10,2μg / ml) amifostine, the cell proliferation inhibition rate and the corresponding concentration of benzene group, the results were significantly different (P <0.01 or P < . Conclusion The inhibitory rate of cell proliferation increased with the increase of the concentration of benzene, and the toxicity of benzene was dose-dependent. Both serum and amifostine could reduce the proliferation toxicity of benzene to cells and could be used as the protective agent of benzene poisoning cells.