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目的研究六亚甲基二乙酰胺(HMBA)对于急性髓性白血病细胞株 HL60细胞的体外作用,并探讨其作用的分子机制。方法 (1)MTT 法检测不同浓度HMBA对于HL60细胞的增殖抑制作用;(2)流式细胞仪(FCM)检测不同浓度HMBA对于HL60细胞表面分化抗原(CD11b、CD14)表达的影响,研究不同浓度 HMBA 对于 HL60细胞
Objective To investigate the in vitro effects of hexamethyldiethanolamine (HMBA) on acute myeloid leukemia cell line HL60 and to explore its molecular mechanism. Methods (1) MTT assay was used to detect the inhibitory effect of HMBA on the proliferation of HL60 cells; (2) Flow cytometry (FCM) was used to detect the effect of different concentration of HMBA on the expression of CD11b and CD14 on HL60 cells; HMBA for HL60 cells